BENEFIT OF SHORTER ABSTINENCE PERIOD ON SPERM DNA INTEGRITY IN CASE OF HIGH LEVEL OF OXIDATIVE STRESS

The objective of this study is to evaluate the influence different periods abstinence on conventional sperm parameters, DNA integrity and oxidative stress. A prospective comparative that was performed between March 2018 2021. total 449 human semen samples were obtained from patients performing an advanced exploration at a private ART clinic. Exclusion criteria included azoospermia. Sperm parameters evaluated according World Health Organization (WHO) 2010 guidelines.In each sample, in addition analysis following measured : • Spermatozoa with strand breaks assessed by TUNEL (cut-off value<30%) Abnormal chromatin condensation using Aniline Blue assays value<20%) Oxidation reduction potential (sORP) MioXSYS Analyzer Patients grouped into 2 groups regard their period sexual (PSA) Group 1 <5 days, ⩾5days. Subgroups defined cutt-off value 1.36mV/106 sperm/mL (1A, 2A) low level sORP (<1.36mV/106 sperm/mL) (1B, 2B) high sORP(>1.36mV/106 SPSS software used for statistical p.value <0.05 considered significant. analysed. Comparable outcomes found group regarding parameters: concentration (38.73 106/ml vs 40.72 106/ml), progressive motility (33% 32%) vitality (62% 60%), p>0.05. When compared same PSA For subgroups days Significant difference observed 1A 1B follwing (52.42 21.66 (37% 26%), (67% 54%), fragmentation (17% 23%), decondensation (20% <0.05. ⩾5days (2A, better results (2A) comparing respectively 2B (56.15 15.82 (36% 22%), (65% 49%), 25%), Best comparison 1B, 2A ; 26% 36% 54% 65% 49), 23% 20% 25 ). In conclusion, our revealed when associated , causes significant impact parameters.